As anticipated, after diminishing contractility, cells responding to uniaxial cues show no significant difference in their ability to sense and protrude along soft and stiff collagen lines (Fig. 2a–e, Supplementary Movie 4), which may be consistent with observations on fibronectin where nascent adhesions replace stable FAs during 1D fibroblast protrusions following myosin inhibition42 and a recent report demonstrating the ability of blebbistatin-treated HT-1080 fibrosarcoma cells to sense fibrous fibronectin-coated guidance cues43. Here, MYH14 is linked to fibrosarcoma.