To verify that the soluble ECD of Lasso, when proteolytically released from the cell-surface as depicted in Figure 2A (Lasso-A), could diffuse between individual cells and bind LPHN1 on distant cells, we co-cultured neuroblastoma cells stably expressing Lasso-A with cells stably expressing LPHN1. The gene discussed is ADGRL1; the disease is neuroblastoma.