Fibroblasts may respond to mechanical loading through a switch to a myofibroblastic phenotype in which they express α‐SMA.32 The transformation of myofibroblasts from CFs is a critical event in the initiation of myocardial fibrosis.33 The total cells were identified using DAPI and vimentin staining to detect the effects of TGD on TGF‐β1–induced myofibroblast transformation. This evidence concerns the gene TGFB1 and Myocardial fibrosis.