RT‐PCR revealed that the expression of mature hepatic genes for proteins involved in biosynthesis (ALB), glycoprotein homeostasis (ASGR2) and metabolic functions (CYP3A7 and CYP3A4) were significantly upregulated in the cells cultured within ICC scaffolds compared with those in 2D (Fig. 5C). This evidence concerns the gene CYP3A7 and intrahepatic cholangiocarcinoma.