Additionally, we have identified 4-(4-nitrobenzyl)-2H-pyrido[3,2-b][1,4]oxazin-3(4H)-one (5e, NPO) can function as potent anticancer agent and demonstrated its potential effects on cell viability, apoptosis, NF-κB activation in HCC cells and tumor growth in preclinical mouse model. Here, NFKB1 is linked to neoplasm.