An elevation in circulating FGF21 was observed in Precon-CH group compared with CH-Con group (P<0.01) and a significant increase in Precon-HF group compared with Naïve-HF (P<0.05) (Figure 7A), suggesting the preconditioning of HF diet increased FGF21 level in the blood. The gene discussed is FGF21; the disease is cyclic hematopoiesis.