CHKA and acute myeloid leukemia: In this study, we analysed the treatment efficacy and potential mechanism of CKI on AML in vitro and in vivo by observing the changes in single-cell morphology using single-cell optofluidics technology, validated the changes in ROS-related targets using Western blot and laser confocal microscopy, and evaluated the treatment effects on AML mice through construction of an AML animal model and AML patient-derived xenograft model.