In peptide immunized 3xTg-AD mice, IFN-γ-secreting cells were detected with 8 ± 11.27 spots in medium control wells and more than 1000 spots in the Aβ42 peptide restimulated wells (p < 0.0001 by Mann-Whitney U test) (Fig. 1f). This evidence concerns the gene IFNG and Alzheimer disease.