In summary, our study convincingly demonstrates that the newly identified ESRP1/circANKS1B/miR-148a/152-3p/USF1 regulatory circuit can induce EMT via the activation of TGF-β1 signaling pathway, thereby contributing to breast cancer invasion and metastasis, meanwhile facilitating the development of new treatment strategy against the metastasis of breast cancer. This evidence concerns the gene ESRP1 and breast carcinoma.