Fig 6A shows strong nuclear staining for Rb S249 phosphorylation in high tumor grade cores LC241c-B2, LC241c-B5, LC241c-C1 and LC241c-C2, all of which are SCC with a tumor grading of 3 and a Rb phospho-S249 staining score of 3. In contrast, Fig 6B shows grade 1 SCC cores LC488-D2 and LC488-E2 that have a Rb phospho-S249 staining score of 0. We also looked for correlations between phospho-Rb T821 staining scores and clinical parameters, but as can be seen in Fig 7, phospho-Rb T821 staining did not correlate with any of these parameters, neither in SCC (Fig 7A) nor in AC (Fig 7B). Here, RB1 is linked to neoplasm.