RB1 and non-small cell lung carcinoma: To investigate this, we conducted a liquid chromatography-tandem mass spectroscopy-based full-length phosphorylation mapping of Rb purified from two non-small cell lung carcinomas (NSCLC) cell lines; H520 cells, which are poorly adhesive and exhibit traits of epithelial-to-mesenchymal (EMT) transition, and H1666 cells, which express epithelial but not EMT-related markers, and have cell-to-cell adhesion.