Direct association between HDAC2 (Histone deacetylase 2), EWSR1 (Ewing sarcoma breakpoint region1), FLI1, SP4/1 and BHLHE40 (Basic helix-loop-helix family member E40) and changes in DNA methylation have not yet been demonstrated but they have been previously associated with changes in histone methylation or acetylation [53–57]. The gene discussed is BHLHE40; the disease is sarcoma.