To quantify the impact of ZIKV infection on the expression levels of key factors during ZIKV infection, which play an instrumental role in determining the osteoblasts phenotype, we quantified gene expression of ALP, Runt-related transcription factor 2 (RUNX2, a key transcription factor for osteoblast differentiation) and a classical inflammatory mediator interleukin 6 (IL6). The gene discussed is RUNX2; the disease is Zika virus infectious disease.