There were no significant differences in the expression levels of sterol regulatory element binding protein (SREBP1)c, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) or stearoyl-CoA desaturase (SCD)1 between the control and MIrs2KO mice, whereas the expression levels of PPARγ, fat-specific protein (FSP)27 and CD36 were significantly increased in the livers of the HF diet-fed MIrs2KO mice (Supplementary Fig. 3f). Here, FASN is linked to hydrops fetalis.