We observed that RACK1 expression was progressively elevated in the carcinogenic process of human colonic epithelium, and was positively correlated with malignant degree and lymph node metastasis of colon cancers, and negatively correlated with patient prognosis; RACK1 enhanced the tumorigenicity of colon cancer cells. The gene discussed is RACK1; the disease is metastatic malignant neoplasm in the lymph nodes.