As senescence is characterized by the irreversible loss of proliferation and alongside apoptosis15–18, we investigated the effects of RACK1 on the proliferation and apoptosis of colon cancer cells, and observed that RACK1 overexpression significantly increased while knockdown significantly decreased colon cancer cell proliferation; RACK1 overexpression significantly decreased while knockdown significantly increased colon cancer cell apoptosis; RACK1 overexpression accelerated G1/S phase progression, whereas RACK1 knockdown blocked cell cycle at G1/S phase in the colon cancer cells. Here, RACK1 is linked to malignant colon neoplasm.