Soft agar colony formation assay showed that RACK1 overexpression significantly increased while knockdown significantly decreased formation ability of soft agar colonies (Fig. 4a), and reexpression of RACK1 in the RACK1 KD colon cancer cells rescued cell anchorage-independent growth (Supplementary Fig. S2), indicating this phenotype not due to off-target effects. The gene discussed is RACK1; the disease is malignant colon neoplasm.