Therefore we examined the protein levels of Beclin 1 (BECN1, a fundamental gene for autophagy induction)30, ATG5, LC3-II (a protein necessary for autophagosome formation) and autophagy-associated protein Sequestosome-1 (SQSTM1) in the colon cancer cell lines with RACK1 expression changes to determine whether RACK1 regulates autophagy of colon cancer cells. This evidence concerns the gene BECN1 and malignant colon neoplasm.