After brain ischemia, 3-Nitro-Trx-1 dramatically increased in vehicle group (Oxy MCAO vs. Oxy, 2.51 ± 0.13 vs. 0.97 ± 0.30, P < 0.05), but significantly reduced in Sev MCAO group (2.51 ± 0.13 vs. 1.58 ± 0.13, Oxy MCAO vs. Sev MCAO, P < 0.05), indicating sevoflurane exerted a down-regulative effect on the nitration of Trx-1 after MCAO. This evidence concerns the gene KMT2A and brain ischemia.