As shown in Fig. 1a, Trx-1 activity in Oxy MCAO group dramatically decreased at 2 h after MCAO (0.64 ± 0.13 vs. 1.18 ± 0.04, Oxy MCAO 2 h vs. Sham, P < 0.05), became even lower at 8 h (0.36 ± 0.04 vs. 0.64 ± 0.13, Oxy MCAO 8 h vs. Oxy MCAO 2 h, P < 0.05), maintained at a low level until 24 h, and then rose up to 72 h after brain ischemia. This evidence concerns the gene KMT2A and brain ischemia.