In a third family (F3), a patient (II.1, P6) with CH and an ectopic thyroid was shown by targeted NGS to have a heterozygous frameshift TUBB1 mutation (c.35delG, p.Cys12Leufs*12, rs77324804) that created a premature stop codon at amino acid 23 (Fig 1). This evidence concerns the gene TUBB1 and cyclic hematopoiesis.