Indeed, an increased EGFR co-localization with the early endosomes marker EEA.1 could be detected in MCF-7 and MDA-MB-231 cells treated with Akt1 siRNA (Fig. 4b), suggesting that PIKfyve activity was inhibited when Akt1 was knocked down in breast cancer cells. The gene discussed is EGFR; the disease is breast carcinoma.