CPT1A and hepatocellular carcinoma: Among the acylcarnitine metabolism-related genes, increased expression of CPT1A (a hepatic isoform of CPT1) and ACSL4 (a member of the ACS family), and decreased expression of CACT and CPT2, were detected in HFD-HCC, indicating that despite the enhanced conversion of FAs to acylcarnitine, the reconversion of acylcarnitine to acyl-CoA was suppressed, which could account for the marked accumulation of acylcarnitine species (Figure 2A).