For example, mRNA and/or protein levels of GSTA1and GSTA4 were increased in liver in response to iron overload [20], and in non-alcoholic fatty liverdisease (NAFLD) progression [21].GSTA1 is an earlier and more sensitive indicator of hepatotoxicity of carbontetrachloride, acetaminophen and ethanol [22]. The gene discussed is GSTA4; the disease is metabolic dysfunction-associated steatotic liver disease.