To emphasize the connection of O‐GlcNAcylation levels, hnRNP‐K and its downstream signals, the expression level of O‐GlcNAcylation, hnRNP‐K, cyclin D1, XIAP, and EMT markers was determined in CCA cell lines, KKU‐100, which shows lower migration activity versus KKU‐213. The gene discussed is XIAP; the disease is cholangiocarcinoma.