To emphasize the connection of O‐GlcNAcylation levels, hnRNP‐K and its downstream signals, the expression level of O‐GlcNAcylation, hnRNP‐K, cyclin D1, XIAP, and EMT markers was determined in CCA cell lines, KKU‐100, which shows lower migration activity versus KKU‐213. This evidence concerns the gene HNRNPK and cholangiocarcinoma.