In order to simulate EMT in vitro, prostate (DU145), head and neck (UP154, UD5) and lung (A549) cancer cells and malignant melanoma (LS174T) cells were incubated with TGFβ for 10 days and with L-lactic-acid (Lac-Ac, 10 mM, pH 6.8) for 2 days. Here, TGFB1 is linked to melanoma.