In order to simulate EMT in vitro, prostate (DU145), head and neck (UP154, UD5) and lung (A549) cancer cells and malignant melanoma (LS174T) cells were incubated with TGFβ for 10 days and with L-lactic-acid (Lac-Ac, 10 mM, pH 6.8) for 2 days. The gene discussed is TGFB1; the disease is cancer.