Furthermore, qRT-PCR analyses indicated that when added in stage 2 (Additional file 1: Figure S5A-F) or stage 3 (Additional file 1: Figure S5G-J), U-46619 enhanced or prolonged Etv2, Gata-2, Tal-1, CD34, and CD31 gene expression but not in the presence of SCH alone or combined SCH/Losma treatment. This evidence concerns the gene TAL1 and spindle cell hemangioma.