For sarcoma, 36% of the cases show CDK4 amplification and protein profiles are discriminable (sdis = − 0.34; p = 0.0; srand = − 6.0e−5) with E-Cadherin, Caveolin-1, Akt_pS473, Cyclin_B1, ER-alpha, Akt_pT308, YAP_pS127, S6_pS240_S244, and Cyclin_E1 decreased and HSP70, Syk, Lck, Src_pY416, and Src_pY527 increased in CDK4 amplified cases. Here, ESR1 is linked to sarcoma.