The sequencing data was analyzed with respect to the three main genes known to be involved in monogenic forms of obesity, i.e. LEP, LEPR and MC4R. Given the high prevalence of LEP, LEPR and MC4R mutations previously reported in Pakistani populations [18], a similarly high prevalence of causal variants within these genes was expected. This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.