RAF1 and infection: Western blot analysis revealed that the level of JEV NS5 protein in infected 293T cells was nearly undetectable at 12 h post-infection (h.p.i.), and it was low at 24 h.p.i. The level of JEV NS5 protein in A549 cells and BHK cells were much more than that in 293T cells at 24 h.p.i. (Figure 1B).