Indeed, by pulling out the gene expression levels of Mrgprx3, Prok2 and Bank1 (all part of the top 20 most variable genes over time list) and combining them 2 by 2 at 3 different time points (expression of Bank1 and Mrgprx3 at 2 hours, expression of Bank1 and Prok2 at 6 hours and expression of Bank1 and Mrgprx3 at 24 hours; see striking image), we could create a tool capable of determining the stroke onset time. This evidence concerns the gene PROK2 and stroke disorder.