In our retrospective study, 72 fetuses with TNF were investigated by karyotype and/or CMA, and the results showed trisomy 21 accounting for 2.8%, Turner syndrome accounting for 2.8%, trisomy 13 accounting for 1.4%, pathogenetic CNVs accounting for 6.9% and other chromosome aberrant accounting for 2.8%. Here, TNF is linked to Turner syndrome.