We hypothesized that similar differences may occur for an individual Ag-specific B cell population within the total B cell compartment, prompting us to employ a system where we could directly monitor defined populations of Ag-specific B cells and CD4 T cells within the total B cell and CD4 T cell compartments to more rigorously study the effect of sepsis on CD4 T cell-dependent B cell responses. The gene discussed is CD4; the disease is Sepsis.