Our results indicated that SB408124 treatment, which subsequently resulted in the inhibition of stimulation of OX1R, induced cell apoptosis in PANC1 cells (Figures 4A,B, right panel, red arrow), whereas cell apoptosis was almost undetectable in orexin-A-treated or non-treated PANC1 cells (Figures 4A,B, left and middle panels, red arrow), suggesting that the regulation of the stimulation of OX1R may be involved in cell apoptosis in pancreatic cancer cells. Here, HCRT is linked to familial pancreatic carcinoma.