To illustrate orexin-A functions in cell proliferation in pancreatic cancer cells, we performed Hoechst staining to confirm whether regulation of the stimulation of OX1R was involved in the induction of cell apoptosis in orexin-A-incubated PANC1 cells with or without SB408124 treatment. The gene discussed is HCRTR1; the disease is familial pancreatic carcinoma.