Co-inertia analysis of IBS patients and healthy subjects based on beta-diversity (JSD) of microbiota composition, revealed separate clusters of the bacterial enterotypes and showed a negative association between the abundance of all fecal granins (CgA, CgB, SgII and SgIII) with SgII as the most important factor contributing to the variation along the first co-inertia component (PC1), (RV = 0.12, p < 0.001; Fig. 5a). This evidence concerns the gene SCG2 and irritable bowel syndrome.