To further delineate the mechanism by which Colchicine induced apoptosis in GC cells, Western blot assay was conducted to detect apoptosis-suppressed protein in NCI-N87 cells, including cleaved caspase-3, Bax, Bcl-2, cytochrome c, PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR. Here, MTOR is linked to gastric cancer.