RBM14 and infection: Since it has been reported that the NS1 proteins of different IAV strains differentially localize to the nucleolus during infection, A549 cells were infected with PR/8, WSN, and Pan/99 viruses at an MOI of 1.0 for 24 h and the cells were fixed and immunostained with anti-RBM14 and anti-NS1 antibodies for imaging by wide-field immunofluorescence microscopy.