All these results indicate that autophagy represents a putative resistance mechanism of colon cancer cell lines bearing mutant BRAFV600E to anti-EGFR mAbs (C, P) and checkpoint inhibitors (NI, PE, IPI) for MSI-H cell line RKO and MEK inhibitors for MSS cell line colo-205, since inhibition of autophagy by 3-MA could sensitize the cells to triple inhibition and apoptosis. This evidence concerns the gene EGFR and colonic neoplasm.