While no studies have previously been published in other cancer cell lines for transient overexpression of TBX4 and 5, numerous studies have shown that transient overexpression of TBX2 or TBX3 does specifically affect senescence by targeting the tumor suppressor gene CDKN1A and B, and CDKN2A (19, 43). The gene discussed is CDKN1A; the disease is cancer.