Bidasee et al. (2001) also reported decreased [3H] ryanodine binding in 6-week post STZ injection, but posited this was due to dysfunctional RyR2 rather than a decrease in protein expression or mRNA level as observed in Teshima et al. (2000) and Choi et al. (2002). Interestingly, Zhao et al. (2014) found that Ca2+ spark frequency showed a gradual decline in correlation with progression of STZ-diabetes, with significant differences between 4-week and 12-week post-injection groups. This evidence concerns the gene RYR2 and diabetes mellitus.