In this study, we examined retinal proteome of the four mouse strains (Akita.PlGF−/−, Akita, C57, and PlGF−/−) using the label-free LC-MS/MS-based proteomics approach in order to understand better the molecular mechanisms that PlGF mediates in the complications of non-proliferative DR (NPDR). Here, PGF is linked to non-proliferative diabetic retinopathy.