Intriguingly, insulin-loaded PAM-PAspPBA-b-PEG has a better effect on preventing the development of hypoglycemia or hyperglycemia than pure insulin in diabetic animals, which suggests that insulin-loaded PAM-PAspPBA-b-PEG might have a better control over blood glucose fluctuation than pure insulin and show a promising application in diabetic individuals. The gene discussed is INS; the disease is Hyperglycemia.