As both PKN3 and p130Cas enhance cell proliferation in vitro and can contribute to various stages of tumor development in vivo (Leenders et al., 2004; Tornillo et al., 2014; Unsal‐Kacmaz et al., 2012) and to expand our results showing in vitro functional relevance of the PKN3–p130Cas interaction to in vivo analysis, we subcutaneously injected SCpkn3−/− cells stably engineered for inducible expression of either PKN3 WT or PKN3 mPR fused to mCherry, or mCherry alone, into nu/nu mice (total 48). Here, BCAR1 is linked to neoplasm.