Genetic ablation of vascular endothelial growth factor (VEGF), a proangiogenic signal protein that is one of the downstream targets of HIF-1 (Figure 1), has been shown to give rise to increases in pimonidazole staining and thus tumor hypoxia on top of reductions in vascularity and tumor volume in a variety of tumors [36, 41–43]. The gene discussed is VEGFA; the disease is neoplasm.