On the other hand, the top 10 statistically significantly mutated genes identified by MutSigCVv.1.4 across the BC samples (n = 1087) in the Pan-Cancer Atlas were PIK3CA (34.7%), TP53 (34.7%), CDH1 (13.3%), GATA3 (12.8%), MAP3K1 (9.1%), PTEN (6.1%), RUNX1 (4.8%), NF1 (4.6%), MAP2K4 (4.4%) and ARID1A (4.3%)65,66. The gene discussed is MAP2K4; the disease is breast cancer.