Further, the expressions of p-PI3K and p-Akt were down-regulated significantly by Shikonin treatment in afatinib-resistant NSCLC cells, demonstrating that Shikonin inhibited cell viability and induced apoptosis of afatinib-resistant NSCLC cells via negative regulation of PI3K/Akt signaling pathway. Here, AKT1 is linked to non-small cell lung carcinoma.