LAT2 and familial pancreatic carcinoma: As shown in Fig. 7a, b, and c, the results indicated that RAD001 could downregulate glutamine synthetase in MIA PaCa-2 and PANC-1 cells and that the levels of both p-mTORSer2448 and glutamine synthetase are decreased in pancreatic cancer cells with CRISPR/Cas9-mediated knockout of LAT2 (LAT2 KO).