In PC-3-EpCAM-KD group, tumour volume was 187.2 ± 24.9 mm3, tumour weight was 0.6 ± 0.1 g; whereas in PC-3-EpCAM-scr group, tumour volume was 304.1 ± 24.2 mm3, tumour weight was 1.2 ± 0.2 g (Mean ± SD, Fig. 2e, P < 0.001 in both measurements), indicating a significant difference in tumour volume and weight in orthotopic model from the two sublines. The gene discussed is EPCAM; the disease is neoplasm.