In PC-3-EpCAM-KD group, tumour volume was 222.3 ± 31.1 mm3, tumour weight was 0.5 ± 0.2 g; whereas in PC-3-EpCAM-scr group, tumour volume was 455.8 ± 44.5 mm3, tumour weight was 1.6 ± 0.1 g (Mean ± SD, Fig. 6b, P < 0.001 in both measurement), suggesting that a significant difference exists in tumour volume and weight between the two sublines. This evidence concerns the gene EPCAM and neoplasm.