To improve the safety of systemic anti-p21Ras scFv delivery for therapy of metastatic and late stage cancers, in this study, we employed CIK cells as a second vector to carry the recombinant adenovirus KGHV500 that harbored the anti-p21Ras scFv gene to tumor foci, and then investigated its anti-colorectal cancer effects. The gene discussed is HRAS; the disease is colorectal cancer.