Collectively, we can conclude that PKC-ζ is elevated in both LoVo and RKO cells, PKC-ι is overexpressed in RKO cell, and the atypical PKC when amplified or mutated are implicated in the metastatic progression of CRC by regulating actin cytoskeleton of CRC cells via Cofilin activation (Figure 10). Here, CFL1 is linked to colorectal carcinoma.