However, Notch-independent functions of RBPJ have also been described.27RBPJ was mutated in 3% of our cohort and homozygously deleted in another case, which is relatively low, but consistent with other forms of cancer.18,28 Importantly, RBPJ alterations were mutually exclusive from NOTCH1 mutations, showed strong trends of association with features of disease progression, and had reduced downstream target expression when considered independently. This evidence concerns the gene NOTCH1 and cancer.