In this study, we investigated the expression of the ESC-associated markers OCT4, SOX2, NANOG, KLF4 and c-MYC in WHO grade I MG using immunohistochemical (IHC) staining, and colorimetric in situ hybridization (CISH) and NanoString mRNA analyses. The gene discussed is POU5F1; the disease is myasthenia gravis.