In the diagnostic process of CRC, BRAF (V600E), PMS2, EGFR, CDX2, CD34, Ki67, P53, MLH1, MSH6, and MSH2 are the most commonly used immunohistochemical markers for pathological typing, differential diagnosis of benign and malignant, and prognosis. The gene discussed is MKI67; the disease is colorectal carcinoma.