By contrast, Wnt/β-catenin pathway has not been extensively studied in ARMS, although Kephart et al. found that an inhibitor of the Wnt/β-catenin pathway secreted frizzled related protein 3 is upregulated in PAX3-FOXO1+ human ARMS cells, suggesting a tumor-suppressive role for Wnt/β-catenin signaling (225). The gene discussed is FOXO1; the disease is alveolar rhabdomyosarcoma.