Recapitulated features of glomerular and tubular diseases by transiently transfecting undifferentiated hPSCs with plasmids expressing wild type Cas9 and sgRNAs targeting disease-relevant genes PODXL (podocalyxin like), polycystic kidney disease (PKD) genes PKD1 and PKD245,46 and TALEN knockout PAX2 to study UB development in vitro16,47. Here, PKD1 is linked to polycystic kidney disease.